Identity and pathogenicity of Botryosphaeriaceae and Diaporthaceae from Juglans regia in Chile

English walnut (Juglans regia) has become an important crop in Chile, representing 11.5% of the total area fruit trees, surpassed only by grapevine. As Chilean industry rapidly expands, young orchards are at risk from emergence new fungal diseases. Botryosphaeriaceae and Diaporthaceae fungi have been recognized as main causes wood diseases walnut, with symptoms dieback, canker, blight. In winter 2017, samples were collected different Valparaíso Maule regions. Fungal isolates recovered cultured, characterized morphologically, identified using DNA sequence analyses. Three species (Neofusicoccum parvum, Diplodia mutila, seriata) two (Diaporthe cynaroidis, Diaporthe australafricana) Diaporthaceae. Pathogenicity tests showed that N. parvum was most aggressive to walnut. This study confirmed presence pathogenic J. regia should be considered increasing for growing industry.